In recent weeks, people have become concerned with the well being of several of the big cats at the Highway 77 Zoo north of Southport.
Florida Fish and Wildlife investigators tell NewsChannel Seven five of the big cats have been put down.
The owner of the Zoo, George Koll, was trying to auction the cats, but could not locate any buyers for the two leopards and three tigers.
State Fish and Wildlife officials got involved trying to place them in refuges in south Florida and across the east coast. When no new home could be found, Koll used a lethal injection to euthanize the animals.
The big cats were bred in captivity; they were not protected under species endangerment laws.