Ramos was sentenced to life in prison 10 years ago for the 1994 shooting death of four people inside the Show n Tail topless night club on Thomas Drive at Panama City Beach.
Two years ago he tried to win a new trial from Circuit Judge Dedee Costello. In his appeal attorneys claimed the original defense attorney failed to adequately represent Ramos.
Judge Costello rejected the claims, so Ramos took his case to the First District Court of Appeals.
Tuesday, the appeals court affirmed Costello's ruling, without comment, so her order denying the new trial stands.
One of the victims in the quadruple murder was the 18-year-old son of now Panama City Mayor Lauren DeGeorge.